产品描述
This Human KLK1 ELISA Kit was designed for the quantitative measurement of Human KLK1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.
别名
Glandular kallikrein 1 ELISA Kit; hK 1 ELISA Kit; hK1 ELISA Kit; Kallikrein serine protease 1 ELISA Kit; Kallikrein-1 ELISA Kit; Kidney/pancreas/salivary gland kallikrein ELISA Kit; Klk 6 ELISA Kit; KLK1 ELISA Kit; KLK1_HUMAN ELISA Kit; Klk6 ELISA Kit; KLKR ELISA Kit; Tissue kallikrein ELISA Kit
缩写
KLK1
Uniprot No
P06870
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 85-103 | |
| 1:2 | Average % | 90 | |
| Range % | 85-99 | |
| 1:4 | Average % | 93 | |
| Range % | 85-97 | |
| 1:8 | Average % | 92 | |
| Range % | 83-98 | |
回收率
| The recovery of human KLK 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 92-101 | |
| EDTA plasma (n=4) | 89 | 85-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.305 | 2.325 | 2.315 | 2.109 | | | 25 | 1.629 | 1.657 | 1.643 | 1.437 | | | 12.5 | 1.124 | 1.139 | 1.132 | 0.926 | | | 6.25 | 0.696 | 0.683 | 0.690 | 0.484 | | | 3.12 | 0.502 | 0.495 | 0.499 | 0.293 | | | 1.56 | 0.355 | 0.342 | 0.349 | 0.143 | | | 0.78 | 0.284 | 0.300 | 0.292 | 0.086 | | | 0 | 0.205 | 0.207 | 0.206 | | |
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货期
3-5 working days