产品描述
This Mouse KITLG ELISA Kit was designed for the quantitative measurement of Mouse KITLG protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
Kitlg; Kitl; Mgf; Sl; Slf; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand
缩写
KITLG
Uniprot No
P20826
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 83 | | |
| Range % | 80-85 | | |
| 1:2 | Average % | 100 | | |
| Range % | 97-103 | | |
| 1:4 | Average % | 86 | | |
| Range % | 83-89 | | |
| 1:8 | Average % | 108 | | |
| Range % | 104-112 | | |
回收率
| The recovery of mouse SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 99 | 95-103 | | |
| EDTA plasma (n=4) | 86 | 83-89 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 1.804 | 1.900 | 1.852 | 1.735 | | | | 100 | 1.195 | 1.201 | 1.198 | 1.081 | | | | 50 | 0.731 | 0.749 | 0.740 | 0.623 | | | | 25 | 0.448 | 0.438 | 0.443 | 0.326 | | | | 12.5 | 0.309 | 0.306 | 0.308 | 0.191 | | | | 6.25 | 0.205 | 0.210 | 0.208 | 0.091 | | | | 3.12 | 0.151 | 0.150 | 0.151 | 0.034 | | | | 0 | 0.118 | 0.116 | 0.117 | | | |
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货期
3-5 working days