产品描述
This Dog MMP-1 ELISA Kit was designed for the quantitative measurement of Dog MMP-1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.18 ng/mL-12 ng/mL and the sensitivity is 0.04 ng/mL.
缩写
MMP-1
灵敏度
0.04 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 85-102 | |
| 1:2 | Average % | 89 | |
| Range % | 85-99 | |
| 1:4 | Average % | 94 | |
| Range % | 85-97 | |
| 1:8 | Average % | 90 | |
| Range % | 83-98 | |
回收率
| The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 82-98 | |
| EDTA plasma (n=4) | 97 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 12 | 2.309 | 2.326 | 2.318 | 2.114 | | | 6 | 1.754 | 1.699 | 1.727 | 1.523 | | | 3 | 1.301 | 1.338 | 1.320 | 1.116 | | | 1.5 | 0.931 | 0.920 | 0.926 | 0.722 | | | 0.75 | 0.573 | 0.585 | 0.579 | 0.375 | | | 0.37 | 0.412 | 0.407 | 0.410 | 0.206 | | | 0.18 | 0.299 | 0.303 | 0.301 | 0.097 | | | 0 | 0.201 | 0.206 | 0.204 | | |
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货期
3-5 working days