产品描述
This Rat KI-67 ELISA Kit was designed for the quantitative measurement of Rat KI-67 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.04 ng/mL.
别名
Nifk ELISA Kit; Mki67ipMKI67 FHA domain-interacting nucleolar phosphoprotein ELISA Kit; Nucleolar protein interacting with the FHA domain of pKI-67 ELISA Kit
缩写
KI-67
Uniprot No
Q5RJM0
灵敏度
0.04 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat KI-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 83-90 | |
| 1:2 | Average % | 93 | |
| Range % | 90-96 | |
| 1:4 | Average % | 89 | |
| Range % | 85-93 | |
| 1:8 | Average % | 100 | |
| Range % | 95-105 | |
回收率
| The recovery of rat KI-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 89 | 85-93 | |
| EDTA plasma (n=4) | 92 | 88-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 10 | 2.796 | 2.731 | 2.764 | 2.566 | | | 5 | 2.447 | 2.407 | 2.427 | 2.229 | | | 2.5 | 1.714 | 1.768 | 1.741 | 1.543 | | | 1.25 | 1.029 | 1.068 | 1.049 | 0.851 | | | 0.625 | 0.614 | 0.638 | 0.626 | 0.428 | | | 0.312 | 0.473 | 0.498 | 0.486 | 0.288 | | | 0.156 | 0.331 | 0.343 | 0.337 | 0.139 | | | 0 | 0.197 | 0.199 | 0.198 | | |
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货期
3-5 working days