产品描述
This Human LT-E4 ELISA Kit was designed for the quantitative measurement of Human LT-E4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.
缩写
LT-E4
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Biochemicals
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human LT-E4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-98 |
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| 1:4 |
Average % |
95 |
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| Range % |
88-100 |
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| 1:8 |
Average % |
98 |
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| Range % |
91-103 |
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回收率
| The recovery of human LT-E4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
85 |
81-93 |
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| EDTA plasma (n=4) |
96 |
93-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.975 |
2.962 |
2.969 |
2.819 |
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| 20 |
2.537 |
2.574 |
2.556 |
2.406 |
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| 10 |
1.646 |
1.769 |
1.708 |
1.558 |
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| 5 |
0.819 |
0.887 |
0.853 |
0.703 |
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| 2.5 |
0.471 |
0.491 |
0.481 |
0.331 |
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| 1.25 |
0.233 |
0.251 |
0.242 |
0.092 |
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| 0.625 |
0.211 |
0.201 |
0.206 |
0.056 |
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| 0 |
0.148 |
0.152 |
0.150 |
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货期
3-5 working days