产品描述
This Hamster IL-6 ELISA Kit was designed for the quantitative measurement of Hamster IL-6 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
缩写
IL-6
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of hamster IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-92 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 96 | |
| Range % | 91-100 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of hamster IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 94-104 | |
| EDTA plasma (n=4) | 91 | 85-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.576 | 2.456 | 2.516 | 2.351 | | | 250 | 2.107 | 2.076 | 2.092 | 1.927 | | | 125 | 1.423 | 1.417 | 1.420 | 1.255 | | | 62.5 | 0.730 | 0.679 | 0.705 | 0.540 | | | 31.2 | 0.457 | 0.470 | 0.464 | 0.299 | | | 15.6 | 0.326 | 0.308 | 0.317 | 0.152 | | | 7.8 | 0.262 | 0.247 | 0.255 | 0.090 | | | 0 | 0.161 | 0.168 | 0.165 | | |
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货期
3-5 working days