产品描述
This Rat IL13 ELISA Kit was designed for the quantitative measurement of Rat IL13 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
别名
Il13 ELISA Kit; Il-13 ELISA Kit; Interleukin-13 ELISA Kit; IL-13 ELISA Kit; T-cell activation protein P600 ELISA Kit
缩写
IL13
Uniprot No
P42203
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 88-93 | |
| 1:2 | Average % | 104 | |
| Range % | 99-107 | |
| 1:4 | Average % | 93 | |
| Range % | 88-97 | |
| 1:8 | Average % | 99 | |
| Range % | 91-103 | |
回收率
| The recovery of rat IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 94 | 91-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 1.854 | 1.766 | 1.810 | 1.727 | | | 250 | 1.117 | 1.106 | 1.112 | 1.029 | | | 125 | 0.728 | 0.743 | 0.736 | 0.653 | | | 62.5 | 0.517 | 0.534 | 0.526 | 0.443 | | | 31.2 | 0.303 | 0.312 | 0.308 | 0.225 | | | 15.6 | 0.210 | 0.202 | 0.206 | 0.123 | | | 7.8 | 0.171 | 0.164 | 0.168 | 0.085 | | | 0 | 0.084 | 0.082 | 0.083 | | |
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货期
3-5 working days