产品描述
MBL2(Mannose-binding lectin 2)是一种由肝脏合成的成分具有免疫调节作用的蛋白质,其能够识别和结合病原体表面的甘露糖。MBL2的正常范围因人而异,但MBL2缺陷与机体免疫功能降低和感染风险增加相关。
华美生物所提供的Human Mannma binding protein/mannan binding lectin,MBP/MBL ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清样本中的MBL2,其灵敏度为0.39 ng/mL,检测范围为1.563 ng/mL-100 ng/mL。
别名
COLEC 1 ELISA Kit; COLEC1 ELISA Kit; Collectin-1 ELISA Kit; HSMBPC ELISA Kit; Lectin; mannose-binding; soluble; 2 ELISA Kit; Mannan binding lectin ELISA Kit; Mannan binding protein ELISA Kit; Mannan-binding protein ELISA Kit; Mannose binding lectin (protein C) 2 soluble ELISA Kit; Mannose binding lectin (protein C) 2; soluble (opsonic defect) ELISA Kit; Mannose binding lectin (protein C) 2; soluble ELISA Kit; Mannose binding lectin 2 soluble ELISA Kit; Mannose binding lectin 2; soluble (opsonic defect) ELISA Kit; Mannose binding lectin ELISA Kit; Mannose binding lectin protein C2 soluble opsonic defect ELISA Kit; Mannose binding protein ELISA Kit; Mannose binding protein C ELISA Kit; Mannose binding protein C precursor ELISA Kit; Mannose binding protein; serum ELISA Kit; Mannose-binding lectin ELISA Kit; Mannose-binding protein C ELISA Kit; MBL 2 ELISA Kit; MBL ELISA Kit; MBL2 ELISA Kit; MBL2_HUMAN ELISA Kit; MBL2D ELISA Kit; MBP 1 ELISA Kit; MBP ELISA Kit; MBP C ELISA Kit; MBP-C ELISA Kit; MBP1 ELISA Kit; MBPB ELISA Kit; MBPC ELISA Kit; MBPD ELISA Kit; MGC116832 ELISA Kit; MGC116833 ELISA Kit; Opsonic defect ELISA Kit; protein C ELISA Kit; Soluble mannose binding lectin ELISA Kit
缩写
MBL2
Uniprot No
P11226
灵敏度
0.171 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MBL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
97 |
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| Range % |
80-106 |
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| 1:200 |
Average % |
93 |
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| Range % |
91-95 |
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| 1:400 |
Average % |
93 |
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| Range % |
85-96 |
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| 1:800 |
Average % |
95 |
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| Range % |
90-101 |
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回收率
| The recovery of human MBL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
93-96 |
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| EDTA plasma (n=4) |
94 |
90-98 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 100 |
1.902 |
1.945 |
1.924 |
1.838 |
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| 50 |
1.720 |
1.596 |
1.658 |
1.572 |
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| 25 |
1.245 |
1.350 |
1.298 |
1.212 |
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| 12.5 |
0.830 |
0.885 |
0.858 |
0.772 |
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| 6.25 |
0.619 |
0.628 |
0.624 |
0.538 |
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| 3.12 |
0.380 |
0.388 |
0.384 |
0.298 |
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| 1.56 |
0.268 |
0.243 |
0.256 |
0.170 |
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| 0 |
0.087 |
0.085 |
0.086 |
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货期
3-5 working days