产品描述
The Chicken Interleukin 18 (IL-18) ELISA KIT is a perfect tool for researchers who are interested in quantifying IL-18 levels in chicken samples,in the field of immunology,inflammatory and infection.This kit is designed to detect and quantify IL-18 in serum, plasma, and tissue homogenates of Gallus gallus (Chicken) samples.
With a detection range of 20 pg/mL-500 pg/mL and a sensitivity of 1.95 pg/mL, this ELISA kit offers high precision and accuracy in measuring IL-18 levels in your samples. The assay time takes only 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength for this ELISA kit is 450 nm, making it a highly sensitive and specific assay. Its sandwich assay principle ensures that you get quantitative measurements with high reproducibility and reliability.
别名
IL18 ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit
缩写
IL18
Uniprot No
Q8QFQ8
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 89-102 | |
| 1:2 | Average % | 90 | |
| Range % | 82-99 | |
| 1:4 | Average % | 93 | |
| Range % | 85-102 | |
| 1:8 | Average % | 96 | |
| Range % | 92-100 | |
回收率
| The recovery of chicken IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 81-99 | |
| EDTA plasma (n=4) | 95 | 89-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 1.981 | 1.927 | 1.954 | 1.851 | | | 250 | 1.334 | 1.374 | 1.354 | 1.251 | | | 125 | 0.920 | 0.915 | 0.918 | 0.815 | | | 62.5 | 0.619 | 0.643 | 0.631 | 0.528 | | | 31.2 | 0.395 | 0.376 | 0.386 | 0.283 | | | 15.6 | 0.290 | 0.306 | 0.298 | 0.195 | | | 7.8 | 0.211 | 0.224 | 0.218 | 0.115 | | | 0 | 0.102 | 0.103 | 0.103 | | |
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货期
3-5 working days
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