产品描述
This Rat MMP14 ELISA Kit was designed for the quantitative measurement of Rat MMP14 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.
缩写
MMP14
Uniprot No
Q10739
灵敏度
11.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-14 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:4 |
Average % |
97 |
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| Range % |
95-107 |
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| 1:8 |
Average % |
101 |
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| Range % |
96-105 |
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回收率
| The recovery of rat MMP-14 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-96 |
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| EDTA plasma (n=4) |
98 |
95-103 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 3000 |
2.288 |
2.289 |
2.289 |
2.092 |
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| 1500 |
1.736 |
1.752 |
1.744 |
1.547 |
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| 750 |
1.118 |
1.097 |
1.108 |
0.911 |
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| 375 |
0.674 |
0.727 |
0.701 |
0.504 |
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| 187.5 |
0.455 |
0.433 |
0.444 |
0.247 |
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| 93.75 |
0.336 |
0.331 |
0.334 |
0.137 |
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| 46.88 |
0.265 |
0.256 |
0.261 |
0.064 |
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| 0 |
0.205 |
0.189 |
0.197 |
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货期
3-5 working days