产品描述

This rat visfatin ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat visfatin in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of visfatin bound in the initial step.

Visfatin (NAMPT) is an adipocytokine that exerts many functions including enhancement of cell proliferation, biosynthesis of nicotinamide mono- and dinucleotide, and hypoglycaemic effect through an endocrine, autocrine, and paracrine manner. Visfatin binds to the insulin receptor at a site different from that of insulin, leading to hypoglycemia by reducing glucose release from liver cells and stimulating glucose utilization in adipocytes and myocytes. Visfatin is upregulated by hypoxia, inflammation, and hyperglycemia but downregulated by insulin, somatostatin, and statins. It is involved in many pathological processes, including obesity, diabetes, and cardiovascular diseases.

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These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 200 1.743 1.867 1.805 1.707 100 1.304 1.199 1.252 1.154 50 0.835 0.838 0.837 0.739 25 0.534 0.547 0.541 0.443 12.5 0.348 0.336 0.342 0.244 6.25 0.277 0.268 0.273 0.175 3.12 0.171 0.177 0.174 0.076 0 0.098 0.097 0.098

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