产品描述

This Human MDK ELISA Kit was designed for the quantitative measurement of Human MDK protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

Amphiregulin associated protein ELISA Kit; Amphiregulin-associated protein ELISA Kit; ARAP ELISA Kit; FLJ27379 ELISA Kit; Mdk ELISA Kit; Midgestation and kidney protein ELISA Kit; Midkine ELISA Kit; MK 1 ELISA Kit; MK ELISA Kit; MK_HUMAN ELISA Kit; MK1 ELISA Kit; NEGF 2 ELISA Kit; NEGF2 ELISA Kit; Neurite growth promoting factor 2 ELISA Kit; Neurite outgrowth promoting protein ELISA Kit; Neurite outgrowth-promoting factor 2 ELISA Kit; Neurite outgrowth-promoting protein ELISA Kit; Retinoic acid inducible factor ELISA Kit

缩写

MDK

Uniprot No

P21741

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		86-97
1:2		Average %		104
		Range %		99-107
1:4		Average %		95
		Range %		88-98
1:8		Average %		98
		Range %		93-103

回收率

The recovery of human MK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		94		89-97
EDTA plasma (n=4)		110		100-113

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   2000 2.686 2.612 2.649 2.532   1000 2.177 2.089 2.133 2.016   500 1.360 1.374 1.367 1.250   250 0.751 0.733 0.742 0.625   125 0.359 0.345 0.352 0.235   62.5 0.296 0.287 0.292 0.175   31.25 0.201 0.191 0.196 0.079   0 0.116 0.118 0.117

货期

3-5 working days