产品描述
This Rat MMP10 ELISA Kit was designed for the quantitative measurement of Rat MMP10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 80 pg/mL-2000 pg/mL and the sensitivity is 50 pg/mL.
别名
Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transformation-associated protein 34A ELISA Kit; Transin-2 ELISA Kit
缩写
MMP10
Uniprot No
P07152
灵敏度
50 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 91 | | |
| Range % | 85-99 | | |
| 1:2 | Average % | 92 | | |
| Range % | 90-103 | | |
| 1:4 | Average % | 95 | | |
| Range % | 87-105 | | |
| 1:8 | Average % | 95 | | |
| Range % | 87-99 | | |
回收率
| The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 96 | 88-100 | | |
| EDTA plasma (n=4) | 95 | 89-102 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | | | | | 2000 | 0.172 | 0.175 | 0.174 | | | | | 800 | 0.376 | 0.379 | 0.378 | | | | | 480 | 0.554 | 0.562 | 0.558 | | | | | 200 | 0.882 | 0.897 | 0.890 | | | | | 80 | 1.493 | 1.475 | 1.484 | | | | | 0 | 2.404 | 2.452 | 2.428 | | | |
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货期
3-5 working days