产品描述
This Mouse LDHA ELISA Kit was designed for the quantitative measurement of Mouse LDHA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL.
别名
Ldha ELISA Kit; Ldh-1 ELISA Kit; Ldh1L-lactate dehydrogenase A chain ELISA Kit; LDH-A ELISA Kit; EC 1.1.1.27 ELISA Kit; LDH muscle subunit ELISA Kit; LDH-M ELISA Kit
缩写
LDHA
Uniprot No
P06151
灵敏度
0.039 mU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDH-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:100 | Average % | 100 | | |
| Range % | 95-106 | | |
| 1:200 | Average % | 88 | | |
| Range % | 84-93 | | |
| 1:400 | Average % | 107 | | |
| Range % | 103-110 | | |
| 1:800 | Average % | 86 | | |
| Range % | 83-90 | | |
回收率
| The recovery of mouse LDH-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 101 | 96-108 | | |
| EDTA plasma (n=4) | 85 | 81-90 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| mU/ml | OD1 | OD2 | Average | | | | | 10 | 0.144 | 0.148 | 0.146 | | | | | 5 | 0.215 | 0.211 | 0.213 | | | | | 2.5 | 0.325 | 0.331 | 0.328 | | | | | 1.25 | 0.508 | 0.499 | 0.504 | | | | | 0.625 | 0.763 | 0.778 | 0.771 | | | | | 0.312 | 1.230 | 1.241 | 1.236 | | | | | 0.156 | 1.561 | 1.570 | 1.566 | | | | | 0 | 2.180 | 2.034 | 2.107 | | | |
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货期
3-5 working days