产品描述

The Human Protein Disulfide Isomerase (PDI) ELISA Kit is an essential tool for quantitative detection of PDI in human serum, plasma, and tissue homogenates. This ELISA kit is specifically designed for research in the field of cancer and cardiovascular, allowing researchers to accurately measure PDI levels with a high level of sensitivity and a wide detection range.

The PDI protein, also known as protein disulfide-isomerase, plays a crucial role in protein folding and disulfide bond formation, making it a key player in various cellular processes. Our PDI ELISA kit is based on a sandwich assay principle, providing reliable and reproducible results with minimal interference from other proteins.

With a sensitivity of 0.078 ng/mL and a detection range of 0.312 ng/mL-20 ng/mL, this ELISA kit can accurately measure PDI levels in a variety of samples. The assay time ranges from 1-5 hours, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, ensuring accurate and precise measurement of PDI levels.

This ELISA kit is an excellent choice for researchers studying cancer and cardiovascular. By providing accurate and sensitive measurements of PDI levels, our ELISA kit can help researchers gain a deeper understanding of the role of PDI in cancer and potentially identify new therapeutic targets.

别名

Cellular thyroid hormone binding protein ELISA Kit; Cellular thyroid hormone-binding protein ELISA Kit; Collagen prolyl 4 hydroxylase beta ELISA Kit; Disulphide Isomerase ELISA Kit; DSI ELISA Kit; EC 5.3.4.1 ELISA Kit; Endoplasmic reticulum resident protein 59 ELISA Kit; ER protein 59 ELISA Kit; ERBA2L ELISA Kit; ERp59 ELISA Kit; GIT ELISA Kit; Gltathione insulin transhydrogenase ELISA Kit; Glutathione insulin transhydrogenase ELISA Kit; P4HB ELISA Kit; P4Hbeta ELISA Kit; p55 ELISA Kit; PDI ELISA Kit; PDIA1 ELISA Kit; PDIA1_HUMAN ELISA Kit; PDIR ELISA Kit; PHDB ELISA Kit; PO4DB ELISA Kit; PO4HB ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) ELISA Kit; Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit ELISA Kit; PROHB ELISA Kit; Prolyl 4 hydroxylase beta polypeptide ELISA Kit; Prolyl 4 hydroxylase beta subunit ELISA Kit; Prolyl 4 hydroxylase subunit beta ELISA Kit; Prolyl 4-hydroxylase subunit beta ELISA Kit; Protein disulfide isomerase associated 1 ELISA Kit; Protein disulfide isomerase, family A, member 1 ELISA Kit; Protein disulfide isomerase/oxidoreductase ELISA Kit; Protein disulfide-isomerase ELISA Kit; Protocollagen hydroxylase ELISA Kit; Thbp ELISA Kit; Thyroid hormone binding protein p55 ELISA Kit; Thyroid hormone binding protein p55 cellular ELISA Kit; V erb a avian erythroblastic leukemia viral oncogene homolog 2 like ELISA Kit

缩写

P4HB

Uniprot No

P07237

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PDI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		95
		Range %		89-99
1:10		Average %		98
		Range %		93-103
1:20		Average %		92
		Range %		88-96
1:40		Average %		95
		Range %		87-99

回收率

The recovery of human PDI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		94-101
EDTA plasma (n=4)		88		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.724 2.587 2.656 2.518 10 2.125 2.008 2.067 1.929 5 1.368 1.397 1.383 1.245 2.5 0.842 0.831 0.837 0.699 1.25 0.526 0.538 0.532 0.394 0.625 0.345 0.334 0.340 0.202 0.312 0.262 0.254 0.258 0.120 0 0.139 0.137 0.138

货期

3-5 working days