产品描述

This Human NFKB1 ELISA Kit was designed for the quantitative measurement of Human NFKB1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

别名

DKFZp686C01211 ELISA Kit; DNA binding factor KBF1 ELISA Kit; DNA binding factor KBF1 EBP1 ELISA Kit; DNA-binding factor KBF1 ELISA Kit; EBP 1 ELISA Kit; EBP-1 ELISA Kit; EBP1 ELISA Kit; KBF1 ELISA Kit; MGC54151 ELISA Kit; nf b ELISA Kit; NF kappa B ELISA Kit; NF kappaB ELISA Kit; NF kappabeta ELISA Kit; NF kB1 ELISA Kit; NF-kappaB ELISA Kit; NFkappaB ELISA Kit; NFKB 1 ELISA Kit; NFKB p105 ELISA Kit; NFKB p50 ELISA Kit; NFKB-p105 ELISA Kit; Nfkb1 ELISA Kit; NFKB1_HUMAN ELISA Kit; Nuclear factor kappa B DNA binding subunit ELISA Kit; Nuclear factor kappa-B ELISA Kit; Nuclear factor kappa-B, subunit 1 ELISA Kit; Nuclear factor NF kappa B p105 subunit ELISA Kit; Nuclear factor NF kappa B p50 subunit ELISA Kit; Nuclear factor NF-kappa-B p50 subunit ELISA Kit; Nuclear factor of kappa light chain gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 ELISA Kit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA Kit; p105 ELISA Kit; p50 ELISA Kit; p84/NF-kappa-B1 p98 ELISA Kit; Transcription factor NFKB1 ELISA Kit

缩写

NFKB1

Uniprot No

P19838

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		89
		Range %		85-94
1:2		Average %		93
		Range %		89-97
1:4		Average %		97
		Range %		91-103
1:8		Average %		90
		Range %		87-96

回收率

The recovery of human NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		85-94
EDTA plasma (n=4)		95		90-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 20 2.889 2.752 2.821 2.710 10 2.251 2.149 2.200 2.089 5 1.399 1.328 1.364 1.253 2.5 0.654 0.643 0.649 0.538 1.25 0.396 0.410 0.403 0.292 0.625 0.241 0.255 0.248 0.137 0.312 0.168 0.181 0.175 0.064 0 0.112 0.110 0.111

货期

3-5 working days