产品描述

This Human LGALS8 ELISA Kit was designed for the quantitative measurement of Human LGALS8 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

别名

Gal 8 ELISA Kit; Gal-8 ELISA Kit; Gal8 ELISA Kit; Galectin 8 ELISA Kit; Galectin-8 ELISA Kit; galectin-8g ELISA Kit; Lectin galactoside binding soluble 8 ELISA Kit; LEG8_HUMAN ELISA Kit; LGAL S8 ELISA Kit; Lgals8 ELISA Kit; PCTA 1 ELISA Kit; PCTA-1 ELISA Kit; PCTA1 ELISA Kit; Po66 carbohydrate binding protein ELISA Kit; Po66 carbohydrate-binding protein ELISA Kit; Po66 CBP ELISA Kit; Po66-CBP ELISA Kit; Prostate carcinoma tumor antigen 1 ELISA Kit

缩写

LGALS8

Uniprot No

O00214

灵敏度

7.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		87-99
1:2		Average %		87
		Range %		83-92
1:4		Average %		90
		Range %		84-93
1:8		Average %		99
		Range %		91-103

回收率

The recovery of human LGALS8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		87		81-93
EDTA plasma (n=4)		95		88-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected     2000 2.012 1.986 1.999 1.826     1000 1.597 1.535 1.566 1.393     500 1.106 1.067 1.087 0.914     250 0.833 0.802 0.818 0.645     125 0.521 0.514 0.518 0.345     62.5 0.384 0.379 0.382 0.209     31.25 0.279 0.272 0.276 0.103     0 0.175 0.171 0.173

货期

3-5 working days