产品描述
This Pig IL15 ELISA Kit was designed for the quantitative measurement of Pig IL15 protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.78 pg/mL-50 pg/mL and the sensitivity is 0.195 pg/mL.
别名
IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写
IL15
Uniprot No
Q95253
灵敏度
0.195 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-89 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 87 | |
| Range % | 84-91 | |
| 1:8 | Average % | 84 | |
| Range % | 81-89 | |
回收率
| The recovery of pig IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 100 | 97-103 | |
| EDTA plasma (n=4) | 84 | 80-88 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 50 | 2.602 | 2.576 | 2.589 | 2.400 | | | 25 | 1.879 | 1.850 | 1.865 | 1.676 | | | 12.5 | 1.144 | 1.168 | 1.156 | 0.967 | | | 6.25 | 0.683 | 0.691 | 0.687 | 0.498 | | | 3.12 | 0.452 | 0.468 | 0.460 | 0.271 | | | 1.56 | 0.361 | 0.391 | 0.376 | 0.187 | | | 0.78 | 0.288 | 0.298 | 0.293 | 0.104 | | | 0 | 0.191 | 0.186 | 0.189 | | |
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货期
3-5 working days