产品描述
This Guinea pig IL13 ELISA Kit was designed for the quantitative measurement of Guinea pig IL13 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.5 pg/mL-300 pg/mL and the sensitivity is 3.75 pg/mL.
缩写
IL-13
Uniprot No
H0W834
灵敏度
3.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 88 | | |
| Range % | 83-92 | | |
| 1:2 | Average % | 95 | | |
| Range % | 85-105 | | |
| 1:4 | Average % | 93 | | |
| Range % | 86-102 | | |
| 1:8 | Average % | 97 | | |
| Range % | 92-110 | | |
回收率
| The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 96 | 91-103 | | |
| EDTA plasma (n=4) | 93 | 86-99 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | | | | | 300 | 2.244 | 2.267 | 2.256 | | | | | 150 | 1.173 | 1.161 | 1.167 | | | | | 75 | 0.628 | 0.650 | 0.639 | | | | | 37.5 | 0.359 | 0.343 | 0.351 | | | | | 15 | 0.152 | 0.151 | 0.152 | | | | | 7.5 | 0.083 | 0.079 | 0.081 | | | |
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货期
3-5 working days