产品描述

This Human KLK10 ELISA Kit was designed for the quantitative measurement of Human KLK10 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 1.675 ng/mL-40 ng/mL and the sensitivity is 0.7 ng/mL.

别名

Breast normal epithelial cell associated serine protease ELISA Kit; Kallikrein related peptidase 10 ELISA Kit; Kallikrein-10 ELISA Kit; Kallikrein10 ELISA Kit; KLK 10 ELISA Kit; KLK10 ELISA Kit; KLK10_HUMAN ELISA Kit; NES 1 ELISA Kit; NES1 ELISA Kit; Normal epithelial cell specific 1 ELISA Kit; Normal epithelial cell-specific 1 ELISA Kit; Protease serine like 1 ELISA Kit; Protease serine-like 1 ELISA Kit; PRSS L1 ELISA Kit; PRSSL 1 ELISA Kit; PRSSL1 ELISA Kit

缩写

KLK10

Uniprot No

O43240

灵敏度

0.7 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		85-106
1:2		Average %		91
		Range %		88-97
1:4		Average %		95
		Range %		90-103
1:8		Average %		95
		Range %		89-101

回收率

The recovery of human KLK 10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		89-100
EDTA plasma (n=4)		95		90-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected 40 1.956 2.014 1.985 1.931 20 1.337 1.324 1.331 1.277 10 0.796 0.808 0.802 0.748 3.3 0.342 0.355 0.349 0.295 1.67 0.211 0.215 0.213 0.159 0 0.053 0.055 0.054

货期

3-5 working days