产品描述

This Human MAGEA3 ELISA Kit was designed for the quantitative measurement of Human MAGEA3 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

Antigen MZ2 D ELISA Kit; Antigen MZ2-D ELISA Kit; Cancer/testis antigen 1.3 ELISA Kit; Cancer/testis antigen family 1 member 3 ELISA Kit; CT1.3 ELISA Kit; HIP8 ELISA Kit; HYPD ELISA Kit; MAGA3_HUMAN ELISA Kit; MAGE 3 antigen ELISA Kit; MAGE family member A3 ELISA Kit; MAGE-3 antigen ELISA Kit; MAGE3 ELISA Kit; MAGEA3 ELISA Kit; MAGEA6 ELISA Kit; Melanoma antigen family A 3 ELISA Kit; Melanoma associated antigen 3 ELISA Kit; Melanoma-associated antigen 3 ELISA Kit; MGC1461 ELISA Kit

缩写

MAGEA3

Uniprot No

P43357

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cancer

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MAGE-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		97
		Range %		89-101
1:2		Average %		95
		Range %		87-99
1:4		Average %		88
		Range %		82-93
1:8		Average %		97
		Range %		91-101

回收率

The recovery of human MAGE-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		104		92-107
EDTA plasma (n=4)		96		88-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 1.742 1.777 1.760 1.642 20 1.383 1.411 1.397 1.279 10 0.860 0.907 0.884 0.766 5 0.539 0.559 0.549 0.431 2.5 0.353 0.344 0.349 0.231 1.25 0.219 0.222 0.221 0.103 0.625 0.175 0.181 0.178 0.060 0 0.117 0.119 0.118

货期

3-5 working days