产品描述

This Human PARP1 ELISA Kit was designed for the quantitative measurement of Human PARP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) ELISA Kit; ADP ribosyltransferase ELISA Kit; ADP ribosyltransferase diphtheria toxin like 1 ELISA Kit; ADP ribosyltransferase NAD(+) ELISA Kit; ADPRT 1 ELISA Kit; ADPRT ELISA Kit; ADPRT1 ELISA Kit; ARTD1 ELISA Kit; msPARP ELISA Kit; NAD(+) ADP ribosyltransferase 1 ELISA Kit; NAD(+) ADP-ribosyltransferase 1 ELISA Kit; pADPRT 1 ELISA Kit; pADPRT-1 ELISA Kit; pADPRT1 ELISA Kit; PARP 1 ELISA Kit; PARP ELISA Kit; PARP-1 ELISA Kit; PARP1 ELISA Kit; PARP1_HUMAN ELISA Kit; Poly (ADP ribose) polymerase 1 ELISA Kit; poly (ADP ribose) polymerase family; member 1 ELISA Kit; Poly (ADP-ribose) polymerase 1 ELISA Kit; Poly [ADP-ribose] polymerase 1 ELISA Kit; Poly(ADP ribose) polymerase ELISA Kit; poly(ADP ribose) synthetase ELISA Kit; poly(ADP ribosyl)transferase ELISA Kit; Poly(ADP-ribosyl)transferase ELISA Kit; Poly[ADP ribose] synthetase 1 ELISA Kit; Poly[ADP-ribose] synthase 1 ELISA Kit; PPOL ELISA Kit; sPARP 1 ELISA Kit; sPARP1 ELISA Kit

缩写

PARP1

Uniprot No

P09874

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PARP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		85
		Range %		81-89
1:2		Average %		92
		Range %		87-96
1:4		Average %		100
		Range %		97-103
1:8		Average %		95
		Range %		91-99

回收率

The recovery of human PARP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		101		97-105
EDTA plasma (n=4)		92		86-99

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.301 2.369 2.335 2.188 500 1.582 1.598 1.590 1.443 250 0.948 0.968 0.958 0.811 125 0.578 0.585 0.582 0.435 62.5 0.389 0.368 0.379 0.232 31.25 0.287 0.264 0.276 0.129 15.6 0.205 0.213 0.209 0.062 0 0.147 0.146 0.147

货期

3-5 working days