产品描述
This Dog IL2 ELISA Kit was designed for the quantitative measurement of Dog IL2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写
IL2
Uniprot No
Q29416
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays):CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 84 | | |
| Range % | 81-89 | | |
| 1:2 | Average % | 95 | | |
| Range % | 92-98 | | |
| 1:4 | Average % | 86 | | |
| Range % | 83-90 | | |
| 1:8 | Average % | 97 | | |
| Range % | 94-100 | | |
回收率
| The recovery of dog IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 85 | 82-90 | | |
| EDTA plasma (n=4) | 105 | 101-109 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.919 | 2.900 | 2.910 | 2.812 | | | | 500 | 2.424 | 2.468 | 2.446 | 2.348 | | | | 250 | 1.670 | 1.620 | 1.645 | 1.547 | | | | 125 | 0.981 | 0.967 | 0.974 | 0.876 | | | | 62.5 | 0.474 | 0.481 | 0.478 | 0.380 | | | | 31.2 | 0.289 | 0.276 | 0.283 | 0.185 | | | | 15.6 | 0.161 | 0.163 | 0.162 | 0.064 | | | | 0 | 0.099 | 0.097 | 0.098 | | | |
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货期
3-5 working days