产品描述

This Human MSMB ELISA Kit was designed for the quantitative measurement of Human MSMB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.5 ng/mL-80 ng/mL and the sensitivity is 1.25 ng/mL .

别名

Beta microseminoprotein [Precursor] ELISA Kit; Beta-microseminoprotein ELISA Kit; HPC 13 ELISA Kit; HPC13 ELISA Kit; IGBF ELISA Kit; Immunoglobulin binding factor ELISA Kit; Immunoglobulin-binding factor ELISA Kit; microseminoprotein beta ELISA Kit; Msmb ELISA Kit; MSMB_HUMAN ELISA Kit; MSP ELISA Kit; MSPB ELISA Kit; PN 44 ELISA Kit; PN44 ELISA Kit; Prostate secreted seminal plasma protein ELISA Kit; Prostate secretory protein of 94 amino acids ELISA Kit; Prostate secretory protein PSP94 ELISA Kit; PRPS ELISA Kit; PRSP ELISA Kit; PSP 57 ELISA Kit; PSP ELISA Kit; PSP-94 ELISA Kit; PSP57 ELISA Kit; PSP94 ELISA Kit; Seminal plasma beta inhibin ELISA Kit; Seminal plasma beta-inhibin ELISA Kit

缩写

MSMB

Uniprot No

P08118

灵敏度

1.25 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Others

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<20%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MSMB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		91
		Range %		85-97
1:2		Average %		96
		Range %		90-102
1:4		Average %		93
		Range %		86-100
1:8		Average %		95
		Range %		86-101

回收率

The recovery of human MSMB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		90-100
EDTA plasma (n=4)		92		88-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average 80 2.243 2.268 2.256 40 1.369 1.352 1.361 20 0.672 0.663 0.668 5 0.204 0.194 0.199 2.5 0.092 0.095 0.094

货期

3-5 working days