产品描述

This Human MYH11 ELISA Kit was designed for the quantitative measurement of Human MYH11 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

AAT4 ELISA Kit; DKFZp686D10126 ELISA Kit; DKFZp686D19237 ELISA Kit; FAA4 ELISA Kit; FLJ35232 ELISA Kit; MGC126726 ELISA Kit; MGC32963 ELISA Kit; MYH 11 ELISA Kit; MYH11 ELISA Kit; MYH11_HUMAN ELISA Kit; Myosin 11 ELISA Kit; Myosin heavy chain 11 ELISA Kit; Myosin heavy chain 11 smooth muscle ELISA Kit; Myosin heavy chain ELISA Kit; Myosin heavy chain smooth muscle isoform ELISA Kit; Myosin heavy polypeptide 11 smooth muscle ELISA Kit; Myosin-11 ELISA Kit; SMHC ELISA Kit; SMMHC ELISA Kit; smooth muscle isoform ELISA Kit; Smooth muscle myosin heavy chain 11 isoform SM2 ELISA Kit; Smooth muscle myosin heavy chain isoform SM2 ELISA Kit

缩写

MYH11

Uniprot No

P35749

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MYH11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		95
		Range %		89-99
1:2		Average %		102
		Range %		97-105
1:4		Average %		98
		Range %		92-101
1:8		Average %		95
		Range %		86-98

回收率

The recovery of human MYH11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		97		94-101
EDTA plasma (n=4)		88		82-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1600 2.295 2.498 2.397 2.258 800 1.543 1.442 1.493 1.354 400 0.891 0.843 0.867 0.728 200 0.528 0.537 0.533 0.394 100 0.322 0.335 0.329 0.190 50 0.255 0.267 0.261 0.122 25 0.174 0.179 0.177 0.038 0 0.138 0.139 0.139

货期

3-5 working days