产品描述
This Mouse LGALS3 ELISA Kit was designed for the quantitative measurement of Mouse LGALS3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
Lgals3 ELISA Kit; Galectin-3 ELISA Kit; Gal-3 ELISA Kit; 35 kDa lectin ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; IgE-binding protein ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Mac-2 antigen ELISA Kit
缩写
LGALS3
Uniprot No
P16110
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GAL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
85-103 |
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| 1:2 |
Average % |
90 |
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| Range % |
82-99 |
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| 1:4 |
Average % |
92 |
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| Range % |
86-100 |
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| 1:8 |
Average % |
92 |
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| Range % |
89-103 |
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回收率
| The recovery of mouse GAL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
90-101 |
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| EDTA plasma (n=4) |
97 |
91-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.852 |
2.842 |
2.847 |
2.703 |
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| 10 |
2.207 |
2.280 |
2.244 |
2.100 |
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| 5 |
1.357 |
1.384 |
1.371 |
1.227 |
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| 2.5 |
0.841 |
0.862 |
0.852 |
0.708 |
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| 1.25 |
0.475 |
0.458 |
0.467 |
0.323 |
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| 0.625 |
0.283 |
0.297 |
0.290 |
0.146 |
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| 0.312 |
0.231 |
0.244 |
0.238 |
0.094 |
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| 0 |
0.142 |
0.146 |
0.144 |
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货期
3-5 working days