产品描述
This Human IL22RA2 ELISA Kit was designed for the quantitative measurement of Human IL22RA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
IL 22BP ELISA Kit; IL 22RA2 ELISA Kit; IL22RA2 ELISA Kit; Class II cytokine receptor ELISA Kit; CRF2 10 ELISA Kit; CRF2 S1 ELISA Kit; CRF2-10 ELISA Kit; CRF2-S1 ELISA Kit; CRF2X ELISA Kit; Cytokine receptor class II member 10 ELISA Kit; Cytokine receptor class-II member 10 ELISA Kit; Cytokine receptor family 2 member 10 ELISA Kit; Cytokine receptor family type 2 ELISA Kit; Cytokine receptor family type 2 soluble 1 ELISA Kit; I22R2_HUMAN ELISA Kit; IL 22 receptor subunit alpha 2 ELISA Kit; IL 22R alpha 2 ELISA Kit; IL-22 receptor subunit alpha-2 ELISA Kit; IL-22BP ELISA Kit; IL-22R-alpha-2 ELISA Kit; IL-22RA2 ELISA Kit; IL22 RA2 ELISA Kit; IL22BP ELISA Kit; IL22R alpha 2 ELISA Kit; Il22ra2 ELISA Kit; Interleukin 22 binding protein ELISA Kit; Interleukin 22 Receptor alpha 2 ELISA Kit; Interleukin-22 receptor subunit alpha-2 ELISA Kit; Interleukin-22-binding protein ELISA Kit; MGC150509 ELISA Kit; MGC150510 ELISA Kit; soluble 1 ELISA Kit; ZcytoR16 ELISA Kit
缩写
IL22RA2
Uniprot No
Q969J5
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL22RA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 94 | | |
| Range % | 87-98 | | |
| 1:2 | Average % | 107 | | |
| Range % | 103-112 | | |
| 1:4 | Average % | 91 | | |
| Range % | 85-97 | | |
| 1:8 | Average % | 92 | | |
| Range % | 88-96 | | |
回收率
| The recovery of human IL22RA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 102 | 98-105 | | |
| EDTA plasma (n=4) | 98 | 93-104 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2000 | 1.927 | 2.057 | 1.992 | 1.889 | | | | 1000 | 1.342 | 1.460 | 1.401 | 1.298 | | | | 500 | 0.922 | 0.965 | 0.944 | 0.841 | | | | 250 | 0.651 | 0.663 | 0.657 | 0.554 | | | | 125 | 0.432 | 0.412 | 0.422 | 0.319 | | | | 62.5 | 0.254 | 0.269 | 0.262 | 0.159 | | | | 31.25 | 0.208 | 0.201 | 0.205 | 0.102 | | | | 0 | 0.104 | 0.102 | 0.103 | | | |
|
货期
3-5 working days