产品描述

This Human MIP-2 ELISA Kit was designed for the quantitative measurement of Human MIP-2 protein in serum, plasma, cell culture supernates, urine. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 7.81 pg/mL.

别名

C-X-C motif chemokine 2 ELISA Kit; Chemokine (C X C motif) ligand 2 ELISA Kit; Chemokine; CXC motif; ligand 2 ELISA Kit; CINC 2a ELISA Kit; CINC2a ELISA Kit; CINC3 ELISA Kit; CXC chemokine ELISA Kit; CXCL 2 ELISA Kit; Cxcl2 ELISA Kit; CXCL2_HUMAN ELISA Kit; Cytokine-induced neutrophil chemoattractant 3 ELISA Kit; GRO 2 ELISA Kit; GRO b ELISA Kit; GRO protein; beta ELISA Kit; Gro-beta ELISA Kit; GRO-beta(5-73) ELISA Kit; GRO-beta-T ELISA Kit; GRO2 ELISA Kit; GRO2 oncogene ELISA Kit; GROb ELISA Kit; GRObeta ELISA Kit; Growth regulated protein beta ELISA Kit; Growth-regulated protein beta ELISA Kit; GROX ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; Macrophage inflammatory protein 2 alpha ELISA Kit; Macrophage inflammatory protein 2 ELISA Kit; Macrophage inflammatory protein 2-alpha ELISA Kit; Melanoma growth stimulatory activity beta ELISA Kit; MGSA b ELISA Kit; MGSA beta ELISA Kit; MIP 2 ELISA Kit; MIP 2a ELISA Kit; MIP2 alpha ELISA Kit; MIP2 ELISA Kit; MIP2-alpha ELISA Kit; MIP2A ELISA Kit; MIP2alpha ELISA Kit; SB-251353 ELISA Kit; SCYB 2 ELISA Kit; Scyb ELISA Kit; SCYB2 ELISA Kit; Small inducible cytokine subfamily B; member 2 ELISA Kit

缩写

MIP-2

Uniprot No

P19875

灵敏度

7.81 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		87-100
1:2		Average %		97
		Range %		91-105
1:4		Average %		100
		Range %		95-106
1:8		Average %		93
		Range %		86-98

回收率

The recovery of human MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		82		80-85
EDTA plasma (n=4)		89		85-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 200 2.352 2.452 2.402 2.276 100 1.812 1.819 1.816 1.690 50 1.174 1.273 1.224 1.098 25 0.703 0.723 0.713 0.587 12.5 0.425 0.428 0.427 0.301 6.25 0.301 0.321 0.311 0.185 3.125 0.201 0.214 0.208 0.082 0 0.125 0.127 0.126

货期

3-5 working days