产品描述
This Human IGJ ELISA Kit was designed for the quantitative measurement of Human IGJ protein in serum, plasma, saliva, urine, tear, breast milk. It is a Sandwich ELISA kit, its detection range is 10 ng/mL-40000 ng/mL and the sensitivity is 10 ng/mL.
别名
IGCJ ELISA Kit; IGJ_HUMAN ELISA Kit; Immunoglobulin J chain ELISA Kit; Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides ELISA Kit; immunoglobulin joining chain ELISA Kit; JCH ELISA Kit; JCHAIN ELISA Kit; Joining chain of multimeric IgA and IgM ELISA Kit
缩写
IGJ
Uniprot No
P01591
灵敏度
10 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays):CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
89 |
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| Range % |
81-99 |
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| 1:2000 |
Average % |
95 |
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| Range % |
83-99 |
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| 1:4000 |
Average % |
95 |
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| Range % |
90-102 |
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| 1:8000 |
Average % |
90 |
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| Range % |
85-100 |
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回收率
| The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
85-99 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40000 |
2.257 |
2.242 |
2.250 |
2.162 |
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| 10000 |
2.154 |
2.138 |
2.146 |
2.058 |
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| 2500 |
1.892 |
1.864 |
1.878 |
1.790 |
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| 625 |
1.252 |
1.249 |
1.251 |
1.163 |
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| 156 |
0.587 |
0.598 |
0.593 |
0.505 |
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| 39 |
0.282 |
0.288 |
0.285 |
0.197 |
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| 10 |
0.141 |
0.139 |
0.140 |
0.052 |
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| 0 |
0.086 |
0.090 |
0.088 |
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货期
3-5 working days