产品描述
白细胞介素6(Interleukin-6,IL-6)是一种由多种细胞产生的细胞因子,包括巨噬细胞、T细胞和B细胞。IL-6参与了人体的多种生理和病理过程,如免疫反应、急性期反应和炎症反应等。IL-6的水平通常在感染、炎症和组织损伤等情况下升高。IL-6也与一些疾病的发生和发展相关,如类风湿关节炎、炎症性肠病和癌症等。
华美生物所提供的Pig Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测猪血清、血浆、组织匀浆样本中的IL6,其灵敏度为0.31 pg/mL,检测范围为1.25 pg/mL-80 pg/mL。
别名
IL6 ELISA Kit; Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL6
Uniprot No
P26893
灵敏度
0.31 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
80-90 |
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| 1:2 |
Average % |
95 |
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| Range % |
91-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
95-103 |
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| 1:8 |
Average % |
90 |
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| Range % |
85-95 |
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回收率
| The recovery of pig IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
85-95 |
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| EDTA plasma (n=4) |
99 |
95-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.212 |
2.101 |
2.157 |
2.066 |
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| 40 |
1.725 |
1.704 |
1.715 |
1.624 |
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| 20 |
1.223 |
1.194 |
1.209 |
1.118 |
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| 10 |
0.871 |
0.809 |
0.840 |
0.749 |
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| 5 |
0.519 |
0.504 |
0.512 |
0.421 |
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| 2.5 |
0.332 |
0.315 |
0.324 |
0.233 |
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| 1.25 |
0.192 |
0.182 |
0.187 |
0.096 |
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| 0 |
0.093 |
0.089 |
0.091 |
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货期
3-5 working days