产品描述

This human nNOS ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human nNOS in multiple samples, including serum, plasma, and cell culture supernates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the nNOS levels in the sample and is measured at 450 nm through a microplate reader.

nNOS, also called NOS1, is predominantly localized in the macula densa, neurons, Bowman's capsule, and collecting duct, where it takes part in the control of glomerular hemodynamics, renin release, and sodium excretion. NOS1 is the predominant nitric oxide (NO)-generating enzyme highly enriched in the brain. It is known to mediate multiple functions, ranging from learning and memory development to maintaining synaptic plasticity and neuronal development, Alzheimer's disease (AD), psychiatric disorders, and behavioral deficits. NOS1 is the major regulator of cardiac function and intracellular Ca2+ fluxes. Considering the physiological actions of NOS1 in the kidney, this enzyme may be involved in the pathogenesis of renal hemodynamic changes associated with diabetes.

别名

2310005C01Rik ELISA Kit; BNOS ELISA Kit; Constitutive NOS ELISA Kit; EC 1.14.13.39 ELISA Kit; IHPS 1 ELISA Kit; IHPS1 ELISA Kit; N-NOS ELISA Kit; NC-NOS ELISA Kit; neuronal Nitric Oxide Synthase ELISA Kit; Neuronal NOS ELISA Kit; Nitric oxide synthase ; neuronal; included ELISA Kit; Nitric oxide synthase 1 (neuronal) ELISA Kit; Nitric oxide synthase 1 ELISA Kit; Nitric oxide synthase; brain ELISA Kit; Nitric oxide synthase; penile neuronal; included ELISA Kit; NNOS ELISA Kit; NO ELISA Kit; NOS 1 ELISA Kit; NOS ELISA Kit; NOS type I ELISA Kit; NOS-I ELISA Kit; NOS1 ELISA Kit; NOS1_HUMAN ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS1 ELISA Kit

缩写

NOS1

Uniprot No

P29475

灵敏度

0.039 IU/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Neuroscience

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human nNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		87
		Range %		82-90
1:10		Average %		105
		Range %		101-110
1:20		Average %		83
		Range %		80-86
1:40		Average %		97
		Range %		91-103

回收率

The recovery of human nNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		89		85-93
EDTA plasma (n=4)		100		95-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml OD1 OD2 Average Corrected 10 2.902 2.963 2.933 2.743 5 2.313 2.365 2.339 2.149 2.5 1.469 1.465 1.467 1.277 1.25 0.878 0.822 0.850 0.660 0.625 0.568 0.545 0.557 0.367 0.312 0.407 0.435 0.421 0.231 0.156 0.314 0.326 0.320 0.130 0 0.192 0.187 0.190

货期

3-5 working days