产品描述
This Rabbit IL1B ELISA Kit was designed for the quantitative measurement of Rabbit IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
别名
IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
缩写
IL1B
Uniprot No
P14628
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 98 | |
| Range % | 85-105 | |
| 1:2 | Average % | 95 | |
| Range % | 85-103 | |
| 1:4 | Average % | 94 | |
| Range % | 82-100 | |
| 1:8 | Average % | 95 | |
| Range % | 85-105 | |
回收率
| The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 82-104 | |
| EDTA plasma (n=4) | 95 | 85-105 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.852 | 2.713 | 2.783 | 2.674 | | | 250 | 2.235 | 2.245 | 2.240 | 2.131 | | | 125 | 1.424 | 1.392 | 1.408 | 1.299 | | | 62.5 | 0.753 | 0.784 | 0.769 | 0.660 | | | 31.2 | 0.447 | 0.433 | 0.440 | 0.331 | | | 15.6 | 0.266 | 0.282 | 0.274 | 0.165 | | | 7.8 | 0.202 | 0.195 | 0.199 | 0.090 | | | 0 | 0.111 | 0.106 | 0.109 | | |
|
货期
3-5 working days