产品描述
OPN (骨钙素,Osteopontin),又称侵袭素,是一种磷酸酸化糖蛋白,广泛分布于多种组织和细胞中,参与骨骼生长、骨代谢、免疫调节、炎症反应、肿瘤发生和转移等生理和病理过程。OPN与许多信号通路和分子相互作用,包括细胞外基质分子、整合素、膜受体、细胞因子、转录因子等,发挥多种生物学效应。OPN的生物活性受到翻译后修饰和剪切变异等因素的影响,其在体内的含量和活性水平也受到多种因素的调节。
华美生物所提供的Human Osteopontin,OPN ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、尿液、组织匀浆样本中的OPN,其灵敏度为0.195 ng/mL,检测范围为0.78 ng/mL-50 ng/mL。
别名
BNSP ELISA Kit; Bone sialoprotein 1 ELISA Kit; Bone sialoprotein I ELISA Kit; BSP I ELISA Kit; BSPI ELISA Kit; Early T lymphocyte activation 1 ELISA Kit; ETA 1 ELISA Kit; ETA1 ELISA Kit; MGC110940 ELISA Kit; Nephropontin ELISA Kit; OPN ELISA Kit; Osteopontin ELISA Kit; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein ELISA Kit; OSTP_HUMAN ELISA Kit; PSEC0156 ELISA Kit; secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1) ELISA Kit; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) ELISA Kit; Secreted phosphoprotein 1 ELISA Kit; SPP 1 ELISA Kit; SPP-1 ELISA Kit; SPP1 ELISA Kit; SPP1/CALPHA1 fusion ELISA Kit; Urinary stone protein ELISA Kit; Uropontin ELISA Kit
缩写
OPN
Uniprot No
P10451
灵敏度
0.195 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV%<10% |
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
94 |
|
| Range % |
88-98 |
|
| 1:10 |
Average % |
100 |
|
| Range % |
94-104 |
|
| 1:20 |
Average % |
93 |
|
| Range % |
88-97 |
|
| 1:40 |
Average % |
99 |
|
| Range % |
93-104 |
|
回收率
| The recovery of human OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
95 |
81-97 |
|
| EDTA plasma (n=4) |
102 |
97-106 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 50 |
2.418 |
2.333 |
2.376 |
2.271 |
|
| 25 |
1.684 |
1.706 |
1.695 |
1.590 |
|
| 12.5 |
1.175 |
1.152 |
1.164 |
1.059 |
|
| 6.25 |
0.654 |
0.664 |
0.659 |
0.554 |
|
| 3.12 |
0.383 |
0.361 |
0.372 |
0.267 |
|
| 1.56 |
0.245 |
0.232 |
0.239 |
0.134 |
|
| 0.78 |
0.176 |
0.185 |
0.181 |
0.076 |
|
| 0 |
0.102 |
0.108 |
0.105 |
|
|
|
货期
3-5 working days