产品描述
This Human ITGA2 ELISA Kit was designed for the quantitative measurement of Human ITGA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.235 ng/mL-15 ng/mL and the sensitivity is 0.058 ng/mL.
别名
BR ELISA Kit; CD 49b ELISA Kit; CD49 antigen like family member B ELISA Kit; CD49 antigen-like family member B ELISA Kit; CD49b ELISA Kit; CD49b antigen ELISA Kit; Collagen receptor ELISA Kit; DX5 ELISA Kit; Glycoprotein Ia deficiency included ELISA Kit; GP Ia ELISA Kit; GP Ia deficiency; included ELISA Kit; GPIa ELISA Kit; HPA 5 included ELISA Kit; HPA5 included ELISA Kit; Human platelet alloantigen system 5 ELISA Kit; Integrin alpha 2 ELISA Kit; Integrin alpha II ELISA Kit; Integrin alpha-2 ELISA Kit; Integrin; alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) ELISA Kit; ITA2_HUMAN ELISA Kit; ITGA2 ELISA Kit; Platelet alloantigen Br(a); included ELISA Kit; Platelet antigen Br ELISA Kit; Platelet glycoprotein GPIa ELISA Kit; Platelet glycoprotein Ia ELISA Kit; Platelet glycoprotein Ia/IIa ELISA Kit; Platelet membrane glycoprotein Ia ELISA Kit; Platelet receptor for collagen; deficiency of; included ELISA Kit; Very late activation protein 2 receptor alpha 2 subunit ELISA Kit; VLA 2 alpha chain ELISA Kit; VLA 2 ELISA Kit; VLA 2 subunit alpha ELISA Kit; VLA-2 subunit alpha ELISA Kit; VLA2 ELISA Kit; VLA2 receptor alpha 2 subunit ELISA Kit; VLAA2 ELISA Kit
缩写
ITGA2
Uniprot No
P17301
灵敏度
0.058 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:5 | Average % | 92 | | |
| Range % | 88-96 | | |
| 1:10 | Average % | 104 | | |
| Range % | 100-107 | | |
| 1:20 | Average % | 92 | | |
| Range % | 89-96 | | |
| 1:40 | Average % | 95 | | |
| Range % | 93-97 | | |
回收率
| The recovery of human ITGA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 98 | 93-104 | | |
| EDTA plasma (n=4) | 93 | 90-96 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 15 | 2.492 | 2.544 | 2.518 | 2.339 | | | | 7.5 | 2.150 | 2.231 | 2.191 | 2.012 | | | | 3.75 | 1.567 | 1.625 | 1.596 | 1.417 | | | | 1.88 | 0.944 | 0.955 | 0.950 | 0.771 | | | | 0.94 | 0.548 | 0.587 | 0.568 | 0.389 | | | | 0.47 | 0.353 | 0.352 | 0.353 | 0.174 | | | | 0.235 | 0.254 | 0.266 | 0.260 | 0.081 | | | | 0 | 0.177 | 0.180 | 0.179 | | | |
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货期
3-5 working days