产品描述
This Pig MMP-9 ELISA Kit was designed for the quantitative measurement of Pig MMP-9 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
缩写
MMP-9
Uniprot No
Q2VI03
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 96 | |
| Range % | 85-106 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 96 | |
| Range % | 88-105 | |
| 1:8 | Average % | 95 | |
| Range % | 90-102 | |
回收率
| The recovery of pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 87-100 | |
| EDTA plasma (n=4) | 97 | 93-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.505 | 2.551 | 2.528 | 2.411 | | | 20 | 2.313 | 2.328 | 2.321 | 2.204 | | | 10 | 1.786 | 1.779 | 1.783 | 1.666 | | | 5 | 1.112 | 1.131 | 1.122 | 1.005 | | | 2.5 | 0.574 | 0.589 | 0.582 | 0.465 | | | 1.25 | 0.364 | 0.374 | 0.369 | 0.252 | | | 0.625 | 0.242 | 0.253 | 0.248 | 0.131 | | | 0 | 0.115 | 0.119 | 0.117 | | |
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货期
3-5 working days