产品描述

This Human neutrophil gelatinase-associated lipocalin (NGAL) ELISA Kit allows for the in vitro quantitative determination of NSE concentrations in serum, plasma, urine, or tissue homogenates. NGAL is produced in kidney tubular cells and then released into the urine by these cells during acute kidney injury (AKI) which is common after heart surgy. Urinary and plasma NGAL has therefore been valuable for the diagnosis and prognosis of AKI. And NGAL can be used to predict the progression of chronic kidney disease (CKD). Ellen F. Carney demonstrated that NGAL) is released by antineutrophil cytoplasmic antibodies (ANCA)-stimulated neutrophils and might have a protective role in ANCA-associated vasculitis (AAV).

Based on the sandwich ELISA technique, NGAL in the sample is sandwiched between pre-coated NGAL antibody and Biotin-conjugated NGAL antibody. The Ag-Ab-Ag immune complex is labeled with HRP-avidin and then develops a color reaction after the addition of the TMB substrate solution. There is a positive correlation between the solution color intensity and the NGAL content in the sample. This kit has many advantages, including a broad detection range, high sensitivity, excellent specificity, and a precision of less than 10%. Get access to more validation details in the product instructions.

别名

24p3 ELISA Kit; 25 kDa alpha 2 microglobulin related subunit of MMP9 ELISA Kit; 25 kDa alpha-2-microglobulin-related subunit of MMP-9 ELISA Kit; Alpha 2 microglobulin related protein ELISA Kit; HGNC:6526 ELISA Kit; HNL ELISA Kit; Lcn 2 ELISA Kit; Lcn2 ELISA Kit; Lipocalin-2 ELISA Kit; Migration stimulating factor inhibitor ELISA Kit; MSFI ELISA Kit; Neutrophil gelatinase associated lipocalin ELISA Kit; Neutrophil gelatinase associated lipocalin precursor ELISA Kit; Neutrophil gelatinase-associated lipocalin ELISA Kit; NGAL ELISA Kit; NGAL_HUMAN ELISA Kit; Oncogene 24p3 ELISA Kit; Oncogenic lipocalin 24p3 ELISA Kit; p25 ELISA Kit; Siderocalin ELISA Kit; siderocalin LCN2 ELISA Kit; SV40 induced 24P3 protein ELISA Kit; Uterocalin ELISA Kit

缩写

NGAL

Uniprot No

P80188

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		84
		Range %		80-88
1:200		Average %		97
		Range %		92-102
1:400		Average %		90
		Range %		88-94
1:800		Average %		93
		Range %		86-98

回收率

The recovery of human NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		90		87-93
EDTA plasma (n=4)		95		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   1000 2.140 2.136 2.138 1.988   500 1.824 1.825 1.825 1.675   250 1.367 1.332 1.350 1.200   125 0.979 1.023 1.001 0.851   62.5 0.510 0.551 0.531 0.381   31.2 0.355 0.372 0.364 0.214   15.6 0.271 0.289 0.280 0.130   0 0.150 0.149 0.150

货期

3-5 working days