产品描述
This Mouse MYH1 ELISA Kit was designed for the quantitative measurement of Mouse MYH1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Myh1 ELISA Kit; Myosin-1 ELISA Kit; Myosin heavy chain 1 ELISA Kit; Myosin heavy chain 2x ELISA Kit; MyHC-2x ELISA Kit; Myosin heavy chain ELISA Kit; skeletal muscle ELISA Kit; adult 1 ELISA Kit
缩写
MYH1
Uniprot No
Q5SX40
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays):CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse sMHC-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
85-99 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-106 |
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| 1:4 |
Average % |
85 |
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| Range % |
81-90 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-108 |
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回收率
| The recovery of mouse sMHC-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
89 |
85-96 |
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| EDTA plasma (n=4) |
87 |
92-93 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.009 |
2.061 |
2.035 |
1.862 |
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| 500 |
1.424 |
1.402 |
1.413 |
1.240 |
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| 250 |
0.980 |
0.951 |
0.966 |
0.793 |
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| 125 |
0.577 |
0.589 |
0.583 |
0.410 |
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| 62.5 |
0.380 |
0.398 |
0.389 |
0.216 |
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| 31.2 |
0.271 |
0.268 |
0.270 |
0.097 |
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| 15.6 |
0.223 |
0.215 |
0.219 |
0.046 |
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| 0 |
0.176 |
0.170 |
0.173 |
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货期
3-5 working days