产品描述
This Dog IL12/P70 ELISA Kit was designed for the quantitative measurement of Dog IL12/P70 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
别名
IL12/P70, Interleukin-12/P70
缩写
IL-12/P70
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 95 | |
| Range % | 91-105 | |
| 1:2 | Average % | 87 | |
| Range % | 84-93 | |
| 1:4 | Average % | 97 | |
| Range % | 91-106 | |
| 1:8 | Average % | 94 | |
| Range % | 86-98 | |
回收率
| The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 87-96 | |
| EDTA plasma (n=4) | 97 | 92-106 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.912 | 2.945 | 2.929 | 2.829 | | | 500 | 2.546 | 2.578 | 2.562 | 2.462 | | | 250 | 1.780 | 1.806 | 1.793 | 1.693 | | | 125 | 1.127 | 1.226 | 1.177 | 1.077 | | | 62.5 | 0.546 | 0.576 | 0.561 | 0.461 | | | 31.2 | 0.259 | 0.269 | 0.264 | 0.164 | | | 15.6 | 0.168 | 0.176 | 0.172 | 0.072 | | | 0 | 0.095 | 0.105 | 0.100 | | |
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货期
3-5 working days