产品描述
This Human IL11 ELISA Kit was designed for the quantitative measurement of Human IL11 protein in serum, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.
别名
Adipogenesis inhibitory factor ELISA Kit; AGIF ELISA Kit; IL 11 ELISA Kit; IL-11 ELISA Kit; Il11 ELISA Kit; IL11_HUMAN ELISA Kit; Interleukin 11 ELISA Kit; Interleukin-11 ELISA Kit; Oprelvekin ELISA Kit
缩写
IL11
Uniprot No
P20809
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-11 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 94-102 | |
| 1:2 | Average % | 90 | |
| Range % | 85-94 | |
| 1:4 | Average % | 95 | |
| Range % | 90-99 | |
| 1:8 | Average % | 89 | |
| Range % | 84-93 | |
回收率
| The recovery of human IL-11 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 84 | 80-88 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.838 | 2.731 | 2.785 | 2.665 | | | 1000 | 2.140 | 2.102 | 2.121 | 2.001 | | | 500 | 1.416 | 1.326 | 1.371 | 1.251 | | | 250 | 0.903 | 0.867 | 0.885 | 0.765 | | | 125 | 0.502 | 0.516 | 0.509 | 0.389 | | | 62.5 | 0.330 | 0.327 | 0.329 | 0.209 | | | 31.25 | 0.264 | 0.253 | 0.259 | 0.139 | | | 0 | 0.119 | 0.121 | 0.120 | | |
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货期
3-5 working days