产品描述

This Human MYD88 ELISA Kit was designed for the quantitative measurement of Human MYD88 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.312ng/mL-20ng/mL and the sensitivity is 0.078 ng/mL.

别名

Mutant myeloid differentiation primary response 88 ELISA Kit; MYD 88 ELISA Kit; Myd88 ELISA Kit; MYD88_HUMAN ELISA Kit; MYD88D ELISA Kit; Myeloid differentiation marker 88 ELISA Kit; Myeloid differentiation primary response 88 ELISA Kit; Myeloid differentiation primary response gene (88) ELISA Kit; Myeloid differentiation primary response gene 88 ELISA Kit; Myeloid differentiation primary response gene ELISA Kit; Myeloid differentiation primary response protein MyD88 ELISA Kit; OTTHUMP00000161718 ELISA Kit; OTTHUMP00000208595 ELISA Kit; OTTHUMP00000209058 ELISA Kit; OTTHUMP00000209059 ELISA Kit; OTTHUMP00000209060 ELISA Kit

缩写

MYD88

Uniprot No

Q99836

灵敏度

0.078 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human MyD88 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-93
1:2		Average %		100
		Range %		97-104
1:4		Average %		99
		Range %		96-102
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human MyD88 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		85		81-90
EDTA plasma (n=4)		92		88-97

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   20 2.488 2.520 2.504 2.331   10 2.045 2.068 2.057 1.884   5 1.381 1.352 1.367 1.194   2.5 0.746 0.788 0.767 0.594   1.25 0.465 0.468 0.467 0.294   0.625 0.352 0.368 0.360 0.187   0.312 0.267 0.251 0.259 0.086   0 0.172 0.174 0.173

货期

3-5 working days