别名
Orm1Alpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
缩写
ORM1
Uniprot No
P02764
灵敏度
3.125 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1000 | Average % | 95 | | |
| Range % | 85-105 | | |
| 1:2000 | Average % | 89 | | |
| Range % | 81-98 | | |
| 1:4000 | Average % | 90 | | |
| Range % | 82-97 | | |
| 1:8000 | Average % | 95 | | |
| Range % | 83-98 | | |
回收率
| The recovery of rat α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 89-102 | | |
| EDTA plasma (n=4) | 95 | 90-99 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | | | | | 800 | 0.213 | 0.217 | 0.215 | | | | | 200 | 0.459 | 0.453 | 0.456 | | | | | 50 | 0.851 | 0.859 | 0.855 | | | | | 12.5 | 1.226 | 1.246 | 1.236 | | | | | 3.125 | 1.727 | 1.740 | 1.734 | | | | | 0 | 1.935 | 1.958 | 1.947 | | | |
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货期
3-5 working days