产品描述

This Human IL23A ELISA Kit was designed for the quantitative measurement of Human IL23A protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

别名

IL 23 A ELISA Kit; IL 23 ELISA Kit; IL 23 subunit alpha ELISA Kit; IL 23A ELISA Kit; IL 23p19 ELISA Kit; IL-23 subunit alpha ELISA Kit; IL-23-A ELISA Kit; IL-23p19 ELISA Kit; IL12B ELISA Kit; IL23 ELISA Kit; Il23a ELISA Kit; IL23A_HUMAN ELISA Kit; IL23P19 ELISA Kit; interleukin 12B ELISA Kit; Interleukin 23 alpha subunit p19 ELISA Kit; Interleukin 23 p19 subunit ELISA Kit; interleukin 23 subunit alpha ELISA Kit; interleukin 23 subunit p19 ELISA Kit; interleukin six; G CSF related factor ELISA Kit; Interleukin-23 subunit alpha ELISA Kit; Interleukin-23 subunit p19 ELISA Kit; JKA3 induced upon T cell activation ELISA Kit; MGC79388 ELISA Kit; P19 ELISA Kit; SGRF ELISA Kit

缩写

IL23A

Uniprot No

Q9NPF7

灵敏度

15.6 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		83-97
1:2		Average %		97
		Range %		91-104
1:4		Average %		84
		Range %		81-88
1:8		Average %		89
		Range %		81-96

回收率

The recovery of human IL-23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		101		94-101
EDTA plasma (n=4)		107		100-114

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 4000 1.762 1.845 1.804 1.628 2000 1.001 1.022 1.012 0.836 1000 0.669 0.667 0.668 0.492 500 0.407 0.416 0.412 0.236 250 0.297 0.277 0.287 0.111 125 0.253 0.246 0.250 0.074 62.5 0.220 0.216 0.218 0.042 0 0.172 0.179 0.176

货期

3-5 working days