产品描述
This Rat Lp-PLA2 ELISA Kit was designed for the quantitative measurement of Rat Lp-PLA2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.78 IU/mL-50 IU/mL and the sensitivity is 0.195 IU/mL.
缩写
Lp-PLA2
Uniprot No
Q5M7T7
灵敏度
0.195 IU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
88-96 |
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| 1:2 |
Average % |
105 |
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| Range % |
100-110 |
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| 1:4 |
Average % |
86 |
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| Range % |
82-90 |
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| 1:8 |
Average % |
93 |
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| Range % |
89-98 |
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回收率
| The recovery of rat Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
90-100 |
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| EDTA plasma (n=4) |
88 |
84-92 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| IU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.203 |
2.361 |
2.282 |
2.152 |
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| 25 |
1.924 |
2.040 |
1.982 |
1.852 |
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| 12.5 |
1.512 |
1.459 |
1.486 |
1.356 |
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| 6.25 |
0.936 |
0.902 |
0.919 |
0.789 |
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| 3.12 |
0.477 |
0.485 |
0.481 |
0.351 |
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| 1.56 |
0.300 |
0.318 |
0.309 |
0.179 |
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| 0.78 |
0.241 |
0.234 |
0.238 |
0.108 |
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| 0 |
0.132 |
0.127 |
0.130 |
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货期
3-5 working days