产品描述
This Rat LT-B4 ELISA Kit was designed for the quantitative measurement of Rat LT-B4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
N/A
缩写
LT-B4
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat LT-B4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 94 | | |
| Range % | 89-99 | | |
| 1:2 | Average % | 97 | | |
| Range % | 91-103 | | |
| 1:4 | Average % | 97 | | |
| Range % | 92-104 | | |
| 1:8 | Average % | 93 | | |
| Range % | 88-97 | | |
回收率
| The recovery of rat LT-B4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 92 | 87-96 | | |
| EDTA plasma (n=4) | 97 | 93-106 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.442 | 2.541 | 2.492 | 2.320 | | | | 500 | 1.887 | 1.986 | 1.937 | 1.765 | | | | 250 | 1.282 | 1.263 | 1.273 | 1.101 | | | | 125 | 0.830 | 0.821 | 0.826 | 0.654 | | | | 62.5 | 0.511 | 0.502 | 0.507 | 0.335 | | | | 31.2 | 0.370 | 0.361 | 0.366 | 0.194 | | | | 15.6 | 0.264 | 0.259 | 0.262 | 0.090 | | | | 0 | 0.171 | 0.172 | 0.172 | | | |
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货期
3-5 working days