产品描述
This Rat MMP3 ELISA Kit was designed for the quantitative measurement of Rat MMP3 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .
别名
Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
缩写
MMP3
Uniprot No
P03957
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:100 | Average % | 90 | |
| Range % | 87-93 | |
| 1:200 | Average % | 94 | |
| Range % | 90-98 | |
| 1:400 | Average % | 95 | |
| Range % | 91-99 | |
| 1:800 | Average % | 92 | |
| Range % | 88-96 | |
回收率
| The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 85 | 81-89 | |
| EDTA plasma (n=4) | 94 | 90-98 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.569 | 2.547 | 2.558 | 2.429 | | | 50 | 2.236 | 2.213 | 2.225 | 2.096 | | | 25 | 1.868 | 1.829 | 1.849 | 1.720 | | | 12.5 | 1.367 | 1.387 | 1.377 | 1.248 | | | 6.25 | 0.840 | 0.889 | 0.865 | 0.736 | | | 3.13 | 0.377 | 0.387 | 0.382 | 0.253 | | | 1.56 | 0.247 | 0.259 | 0.253 | 0.124 | | | 0 | 0.128 | 0.130 | 0.129 | | |
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货期
3-5 working days