产品描述

This Human KRT23 ELISA Kit was designed for the quantitative measurement of Human KRT23 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 ng/mL-60 ng/mL and the sensitivity is 0.23 ng/mL.

别名

CK 23 ELISA Kit; CK-23 ELISA Kit; CK23 ELISA Kit; Cytokeratin 23 ELISA Kit; Cytokeratin-23 ELISA Kit; Cytokeratin23 ELISA Kit; DKFZP434G032 ELISA Kit; HAIK 1 ELISA Kit; HAIK1 ELISA Kit; Histone deacetylase inducible keratin 23 ELISA Kit; Hyperacetylation inducible type I keratin ELISA Kit; K1C23_HUMAN ELISA Kit; K23 ELISA Kit; Keratin 23 (histone deacetylase inducible) ELISA Kit; Keratin 23 ELISA Kit; Keratin ELISA Kit; Keratin type I cytoskeletal 23 ELISA Kit; Keratin-23 ELISA Kit; Keratin23 ELISA Kit; KRT 23 ELISA Kit; KRT23 ELISA Kit; MGC26158 ELISA Kit; type I cytoskeletal 23 ELISA Kit; Type I intermediate filament cytokeratin ELISA Kit

缩写

KRT23

Uniprot No

Q9C075

灵敏度

0.23 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KRT23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		86
		Range %		81-92
1:2		Average %		91
		Range %		87-96
1:4		Average %		88
		Range %		82-94
1:8		Average %		92
		Range %		86-98

回收率

The recovery of human KRT23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		96		91-102
EDTA plasma (n=4)		93		87-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 60 2.761 2.562 2.662 2.537 30 2.155 2.062 2.109 1.984 15 1.337 1.305 1.321 1.196 7.5 0.745 0.720 0.733 0.608 3.75 0.413 0.405 0.409 0.284 1.8 0.276 0.283 0.280 0.155 0.9 0.193 0.189 0.191 0.066 0 0.125 0.124 0.125

货期

3-5 working days