产品描述
This Rat PCSK9 ELISA Kit was designed for the quantitative measurement of Rat PCSK9 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 46.88 pg/mL.
别名
Pcsk9 ELISA Kit; Narc1 ELISA Kit; Proprotein convertase subtilisin/kexin type 9 ELISA Kit; EC 3.4.21.- ELISA Kit; Neural apoptosis-regulated convertase 1 ELISA Kit; NARC-1 ELISA Kit; Proprotein convertase 9 ELISA Kit; PC9 ELISA Kit; Subtilisin/kexin-like protease PC9 ELISA Kit
缩写
PCSK9
Uniprot No
P59996
灵敏度
46.88 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PCSK9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
92 |
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| Range % |
82-98 |
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| 1:200 |
Average % |
93 |
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| Range % |
87-96 |
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| 1:400 |
Average % |
98 |
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| Range % |
92-104 |
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| 1:800 |
Average % |
87 |
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| Range % |
82-95 |
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回收率
| The recovery of rat PCSK9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
89-97 |
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| EDTA plasma (n=4) |
98 |
96-104 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 6000 |
0.088 |
0.087 |
0.088 |
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| 3000 |
0.185 |
0.188 |
0.187 |
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| 1500 |
0.358 |
0.369 |
0.364 |
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| 750 |
0.624 |
0.630 |
0.627 |
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| 375 |
1.130 |
1.111 |
1.121 |
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| 187.5 |
1.516 |
1.500 |
1.508 |
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| 93.75 |
1.810 |
1.712 |
1.761 |
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| 0 |
2.169 |
2.064 |
2.117 |
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货期
3-5 working days