产品描述
This Human NDP ELISA Kit was designed for the quantitative measurement of Human NDP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.
别名
EVR2 ELISA Kit; Exudative vitreoretinopathy 2 (X linked) ELISA Kit; FEVR ELISA Kit; ND ELISA Kit; NDP ELISA Kit; NDP_HUMAN ELISA Kit; Norrie disease (pseudoglioma) ELISA Kit; Norrie disease protein ELISA Kit; Norrin ELISA Kit; Norrin precursor ELISA Kit; X linked exudative vitreoretinopathy 2 protein ELISA Kit; X-linked exudative vitreoretinopathy 2 protein ELISA Kit
缩写
NDP
Uniprot No
Q00604
灵敏度
0.078 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human NDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 102 | |
| Range % | 96-105 | |
| 1:2 | Average % | 99 | |
| Range % | 92-103 | |
| 1:4 | Average % | 90 | |
| Range % | 82-94 | |
| 1:8 | Average % | 99 | |
| Range % | 92-103 | |
回收率
| The recovery of human NDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 82-95 | |
| EDTA plasma (n=4) | 104 | 95-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.619 | 2.545 | 2.582 | 2.444 | | | 10 | 2.079 | 2.017 | 2.048 | 1.910 | | | 5 | 1.384 | 1.399 | 1.392 | 1.254 | | | 2.5 | 0.733 | 0.754 | 0.744 | 0.606 | | | 1.25 | 0.530 | 0.521 | 0.526 | 0.388 | | | 0.625 | 0.278 | 0.301 | 0.290 | 0.152 | | | 0.312 | 0.207 | 0.212 | 0.210 | 0.072 | | | 0 | 0.139 | 0.137 | 0.138 | | |
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货期
3-5 working days