产品描述

This Human KRT20 ELISA Kit was designed for the quantitative measurement of Human KRT20 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

别名

CD20 ELISA Kit; CK 20 ELISA Kit; CK-20 ELISA Kit; CK20 ELISA Kit; Cytokeratin-20 ELISA Kit; Cytokeratin20 ELISA Kit; K1C20_HUMAN ELISA Kit; K20 ELISA Kit; KA20 ELISA Kit; Keratin 20 ELISA Kit; keratin 20, type I ELISA Kit; keratin 21, rat, homolog of ELISA Kit; Keratin ELISA Kit; Keratin type I cytoskeletal 20 ELISA Kit; Keratin-20 ELISA Kit; Keratin20 ELISA Kit; KRT 20 ELISA Kit; KRT 21 ELISA Kit; KRT20 ELISA Kit; KRT21 ELISA Kit; MGC35423 ELISA Kit; OTTHUMP00000164518 ELISA Kit; Protein IT ELISA Kit; type I cytoskeletal 20 ELISA Kit

缩写

KRT20

Uniprot No

P35900

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human CK20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		103
		Range %		96-108
1:2		Average %		86
		Range %		80-92
1:4		Average %		95
		Range %		90-100
1:8		Average %		94
		Range %		89-99

回收率

The recovery of human CK20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		88-97
EDTA plasma (n=4)		96		90-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.766 2.868 2.817 2.673 20 2.101 1.997 2.049 1.905 10 1.172 1.244 1.208 1.064 5 0.725 0.732 0.729 0.585 2.5 0.363 0.387 0.375 0.231 1.25 0.266 0.284 0.275 0.131 0.625 0.228 0.246 0.237 0.093 0 0.144 0.143 0.144

货期

3-5 working days