产品描述
This Bovine ORM1 ELISA Kit was designed for the quantitative measurement of Bovine ORM1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 62.5 ng/mL-4000 ng/mL and the sensitivity is 15.6 ng/mL.
别名
ORM1 ELISA kit; AGPAlpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
缩写
ORM1
Uniprot No
Q3SZR3
灵敏度
15.6 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
102 |
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| Range % |
94-106 |
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| 1:2000 |
Average % |
92 |
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| Range % |
84-97 |
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| 1:4000 |
Average % |
101 |
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| Range % |
95-104 |
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| 1:8000 |
Average % |
97 |
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| Range % |
92-101 |
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回收率
| The recovery of bovine α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
88-99 |
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| EDTA plasma (n=4) |
92 |
88-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.229 |
0.225 |
0.227 |
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| 2000 |
0.450 |
0.459 |
0.455 |
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| 1000 |
0.698 |
0.656 |
0.677 |
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| 500 |
1.016 |
0.987 |
1.002 |
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| 250 |
1.264 |
1.243 |
1.254 |
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| 125 |
1.572 |
1.514 |
1.543 |
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| 62.5 |
1.671 |
1.634 |
1.653 |
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| 0 |
1.815 |
1.789 |
1.802 |
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货期
3-5 working days